ACS Now Offers Quantitative Endotoxin Testing for Peptides
We're pleased to announce that quantitative endotoxin testing via LAL (Limulus Amebocyte Lysate) assay is now available at ACS Peptide Testing Labs. This addition completes our comprehensive contaminant testing panel alongside heavy metal screening (ICP-MS) and residual solvent analysis (GC-MS).
Endotoxin testing has been one of our most requested services. With growing concerns about what's really in peptides -- especially those intended for injection or compounding -- we've invested in the Charles River Endosafe nexgen-PTS system to deliver quantitative results with industry-leading sensitivity.
Why Endotoxin Testing Matters
Endotoxins are lipopolysaccharides (LPS) released from the cell walls of gram-negative bacteria. What makes them particularly dangerous:
- Heat-stable: Standard autoclaving and sterilization do NOT eliminate endotoxins
- Ubiquitous: Present in water, on surfaces, and in manufacturing environments
- Potent: Even trace amounts (nanogram levels) can trigger fever, inflammation, and septic shock
- Invisible: Cannot be detected by visual inspection, smell, or standard sterility testing
For injectable peptides, endotoxin contamination is arguably the most critical safety concern. The FDA requires all injectable products to meet USP endotoxin limits -- and for good reason.
What We've Seen in Our Lab
At ACS Peptide Testing Labs, we've tested over 1,400 peptide samples since launch. Our data reveals concerning trends:
- 17% of peptide samples contain detectable heavy metals above USP limits
- Many samples from online suppliers arrive in containers that are not certified endotoxin-free
- Peptides reconstituted in non-sterile environments frequently show elevated endotoxin levels
- 73% of peptides show purity discrepancies vs. supplier claims
These findings reinforce why comprehensive contaminant testing -- including endotoxins -- is essential, not optional.
Our Endotoxin Testing Service
Equipment & Method
We use the Charles River Endosafe nexgen-PTS system with the kinetic chromogenic LAL method. This provides:
- Quantitative results (not just pass/fail)
- Sensitivity: 0.01 EU/mL
- USP <85> compliant methodology
- Rapid turnaround: 9-11 business days
Endotoxin Limits by Application
| Application | USP Limit |
|---|---|
| Injectable peptides | Less than 5 EU/kg body weight |
| Intrathecal use | Less than 0.2 EU/kg body weight |
| Cell culture research | Less than 0.5 EU/mL recommended |
| General research use | Less than 20 EU/mL typical specification |
Pricing
- Standalone endotoxin testing: $150-$300 per sample
- Comprehensive contaminant panel (endotoxin + heavy metals + residual solvents): Bundled pricing available
- Volume discounts: 25-35% off for 5+ samples
Request a quote for your specific needs.
